Lugol's Solution (B) for Gram Staining

Lugol's Solution (B) is a solution used as a mordant (fixative) in the second step of the Gram staining protocol. This solution binds the Crystal Violet dye more strongly to the cell walls of Gram-positive bacteria, clarifying the distinction between Gram-positive and Gram-negative bacteria. It is indispensable for the fixation process, which is an important step in Gram staining processes.

Features

• Mordant Effect: Provides better fixation of Crystal Violet dye on Gram-positive cell walls.

• Sensitive Formulation: Provides reliable results without damaging bacterial cells.

• Compatible and Stable: Compatible with all Gram staining protocols and maintains its effectiveness for a long time.

• Easy Usage: It provides ease of use by being presented in ready-made solution form.

• High Purity: High purity formula suitable for microbiological analysis.

Areas of Use

• Gram Staining Protocols:
  It is used as a fixative to ensure that Gram-positive bacteria retain the Crystal Violet stain.

• Microbiological Diagnosis:
  It is preferred in medical and clinical laboratories to perform Gram classification of infectious agents.

• Education and Research:
  Used in microbiology courses and research projects to apply Gram staining techniques.

• Food and Water Microbiology:
  Applied for Gram classification of microorganisms in food and water samples.

Storage Conditions

• Should be stored at room temperature between 15-25°C.

• Should be protected from direct sunlight and high humidity.

• After use, the lid should be tightly closed and stored in a cool environment.

• ÇShould be kept out of reach of children and away from sources of fire/sparks.