Cell Culture Compatible SBF (Cell Culture Compatible)

Cell Culture Compatible SBF

Simulated Body Fluid for Pre-Cellular Mineralization – ISO 10993-5 Compliant

Testonic® Cell Culture Compatible SBF is a non-cytotoxic simulated body fluid formulated in accordance with ISO 10993-5 for use in pre-cell culture mineralization studies. It is designed to allow calcium-phosphate deposition on biomaterial surfaces without harming cells, offering stable ionic composition and physiological pH through an optimized buffering system.

Application Areas

• Pre-conditioning of biomaterial surfaces before cell seeding

• Evaluation of hydroxyapatite formation without cytotoxicity

• Biocompatibility testing combined with surface mineralization

• In vitro bioactivity studies under cell culture-compatible conditions

• Preparation step prior to serum-containing media incubation

Formulation Features

Product Features

• Specially designed for use before cell culture

• Supports apatite layer formation without harming living cells

• Maintains cell adhesion and proliferation compatibility

• Clear, particle-free and sterilizable formulation

Usage & Storage Instructions

• Bring to room temperature before use

• In CO₂ incubators, HCO₃⁻ buffering activates automatically

• In CO₂-free setups, HEPES buffer maintains pH stability

• Store at 2–8 °C, do not freeze

Why Choose Testonic® Cell Culture Compatible SBF?

• Fully compliant with ISO 10993-5

• Allows surface mineralization without cellular toxicity

• Dual-buffer system (HEPES + bicarbonate) ensures pH stability

• Contains glucose for compatibility with culture environments